EN
Zonke imidlalo

Ikhaya>Izindaba>Ukunakekelwa Kwempilo


Ngabe luteolin yenzani kulesimila?

Usuku: 2019-10-17

I-anti-tumor fuction

I-1.1 Vimbela ukwanda kwamaseli we-tumor

Umphumela we-inhibitory we-Luteolin kuma-tumor cell proliferation ngokuyinhloko uvimbela umsebenzi wama-kinases athile esitokisini kanye nokuboshwa komjikelezo weseli. U-Aurora B kinase, ilungu lomndeni wakwa-Aurora kinase, uyi-protein serine / threonine kinase ethinteka emicimbini eminingi ye-mitotic futhi ungumlawuli obalulekile we-mitotic. I-Histone H3 i-substrate ethile ye-Aurora B kinase, kanye ne-phosphorylation kwaSer10 ibonisa umsebenzi we-Aurora B kinase. U-Xie et al. Uveze ukuthi ngemuva kokuphathwa kwe-Luteolin, i-phosphorylated histone H3 (Ser10) okuqukethwe kwamaprotheni ku-HeLa namaseli we-SW20 kwehle, futhi okuqukethwe kwamaprotheni we-Aurora B kinase ne-histone H3 ephelele yahlala ingashintshiwe. Lokhu kukhombisa ukuthi i-Luteolin ivimbela ukwanda kwamaseli we-HeLa nama-SW20 ngokuthinta umsebenzi we-laser kinase B ngaphandle kokuguqula okuqukethwe kwawo. Attrib et al. Ucwaningo lufakazele ukuthi iLuteolin inyuse ukuvezwa kwamaprotheni e-histone H3 ne-H4 acetylated kumaseli we-LNM35, futhi njenge-inhibitor ye-histone deacetylase, yavimbela ukwanda kwamaseli we-LNM35. Ukuthi inqubo eyi-histone deacetylase inhibitors inhibit cell proliferation ayiqondakali kahle. Ucwaningo lukhombisile ukuthi ukuvimbela i-histone deacetylation kunganciphisa imfoloko ephindaphindekayo, kusebenze ukungasebenzi kahle futhi kuholele ekulimaleni kwe-DNA.
ILuteolin nayo ingavimba umjikelezo weseli. U-Lee et al.research ukhombise ukuthi uLuteolin unyuke kakhulu nge-estrogen receptor (i-ER) ye-serine-threonine kinase (AKT) kanye nomugqa weseli womdlavuza webele wesibeletho i-MDA-MB-231 negative 、 Polo-like kinase 1 , PLK1 、 cyclin B1 、 cyclin Umjikelezo we-cell 、 cell division 2 (CDC2) 、 kanye ne-cyclin-wate kinase 2 (CDK2) njll. Futhi wehlise okuqukethwe kwamaprotheni kwe-proteinjikelezo lomjikelezo we-protein kinase inhibitor p21, futhi wenze umjikelezo weseli umile ku-G2 / M no-S. Uma isetshenziswa ihlanganiswe ne-genermal ukukhula factor (EGF), iLuteolin ingathola i-EGF ebangwa yi-PI3K / AKT kanye ne-mitogen-activated protein kinase (MAPK) indlela yokuqalisa ukusebenza, okwenza i-EGFR 、 AKT 、 p38 kanye ne-extracellular signal-regature kinase (ERK) phosphorylation ukuvimbela ukusinda kweseli kwe-EGFR. UShoulars et al. kusetshenziswa ubuchwepheshe be-chromatin immunoprecipitation-gene chip, kusonge umphumela we-Luteolin kuphrofayili yokuveza uhlobo lweseli lomdlavuza wendlala yesibindi ye-PC-3 cell. Kutholakale ukuthi inkulumo ekhulayo yohlobo lwe-PLK1, eyandisa iseli ye-antigen (PCNA) ne-cyclin E2 (CCNE2) ebandakanyeka kumjikelezo weseli yenyuka, futhi isisho se-kinase inhibitor 1B, CDKN1B) sincishisiwe futhi lezi zinguquko zaqinisekiswa ku izinga lamaprotheni yi-Western blot.



I-1.2 Induction apoptosis yeseli lesisu

I-Luteolin ingayenga i-apoptosis yamaseli wesimila ngokusebenzisa indlela ye-mitochondrial apoptosis, ukucindezela kwe-endoplasmic reticulum, kanye nendlela yokufa ye-receptor apoptosis.
Choi et al. wasebenzisa amaseli wegundane lobuchopho (i-Neuro-2a) njengemodeli yeseli ukucubungula inqubo ye-Luteolin-indosed apoptosis. Ngemuva kokuthola isenzo se-luteolin, inkulumo ye-apoptosis ye-intracellular ekhuthaza amaprotheni i-Bax ne-Bim yanda, futhi inkulumo ye-anti-apoptotic protein Bcl-2 yancipha. Njengoba isikhathi siqhubeka, i-cytochrome
I-C ku-mitochondria iyancipha kancane kancane, ngenkathi i-cytochrome C ku-cytoplasm ikhuphuka kakhulu, kanti ne-caspase-fulani proteinases caspase-3, i-Caspase-9, i-caspase-12 ne-poly (ADP-ribose) polymerase, i-PARP ikhuphuka ngokuqina okukhona. I-Luteolin isebenzisa indlela ye-mitochondrial apoptotic. Ucwaningo olwengeziwe luye lwabonisa ukuthi i-luteolin ithinta amaprotheni ama-endoplasmic reticulum aphathelene nokukhathazeka futhi inyusa amaprotheni e-CCAAT / ama-protein enzalo (CHOP) 、 (glucose- Ukulawulwa kwamaprotheni, i-GRP) i-94 ne-78, njll. I-ATF6cy. Indlela ye-mitochondrial apoptotic pathway kanye ne-endoplasmic reticulum ingcindezi ayihlukanisiwe komunye nomunye. I-plpidid yokuphazanyiswa kwe-Caspase-6 inganciphisa ukuvezwa kwe-Caspase-12 ne-CHOP, kanti ne-CHOP ukuphazanyiswa kwe-plasmid nakho kunganciphisa okuqukethwe kwe-caspase-3 I-Luteolin isebenza ngaphakathi kwe-12 min yeseli, futhi amaprotheni amathathu womzila we-MAPK, i-ERK, i-p10 kanye ne-c-Jun N terminal kinase (JNK) icushiwe futhi i-phosphorylated, futhi i-MAPK inhibitor yehlisa inani le-cytochrome C kuBax naku-cytoplasm. . Ngokufanayo, esikhathini esifushane, izinhlobo ze-oksijini ezisebenzayo (i-ROS) nazo zavela emangqamuzaneni, futhi ama-ROS inhibitors awakwazi ukuguqula izinguquko ze-MAPK, futhi azange athinte amandla okuqala we-mitochondrial membrane, kodwa angakwazi ukuthola amandla we-membochane we-mitochondrial sekwedlule isikhathi. Lokhu kuphakamisa ukuthi i-Luteolin ngokusebenzisa kusebenze i-MAPK ngokwenza okokuqala i-Bax yenzeke i-mitochondrial translocation, okuholela ekungasebenzi kahle kwangaphambi kokusebenza kwe-mitochondrial, ikhiqize i-ROS yokuqala nokucindezela kwe-reticulum yangaphambi kwesikhathi. Ukungasebenzi kahle kwe-mitochondrial kungakhulisa ukukhiqizwa kwe-ROS futhi kuholele ekucindezelekeni kwe-endoplasmic reticulum. Ukucindezelwa kwe-endoplasmic reticulum kanye ne-mitochondrial apoptosis kungagcina kusebenze ukusabela kwe-Caspase Cascade kanye ne-Indize cell apoptosis.



U-Lee et al. wasebenzisa ukuhlaziywa kwe-proteinomic ukukhombisa ukuthi u-Luteolin wenza i-apoptosis yamaseli e-CH27 kwaba inqubo ye-mitochondria ne-endoplasmic reticulum isebenza ndawonye, ​​futhi i-ATP yayibandakanyeka. Esigabeni sokuqala se-apoptosis, i-Luteolin ikhipha ukukhishwa kwe-ATP futhi okuqukethwe kuyanda; esigabeni sokugcina se-apoptosis, i-α-enolase (α-enolase) ne-hydroxyalkyl-coenzyme A dehydrogenase (ATP synthase) eyonakele nokuqukethwe kwe-ATP kuyancipha.
I-Luteolin ingabuyisela i-apoptosis yeseli ngomgudu wokufa we-receptor. Emshinini wokufunda nge-luteolin ngamaseli e-HeLa, kwatholakala ukuthi i-Caspase inhibitor zVAD-fmk kanye ne-caspase-10 inhibitor zAEVD-fmk kakhulu ukuvimbela ukufa kweseli kwe-Luteolin. Ezingeni lokubhalwa kokubhaliwe, i-luteolin ikhuphule kakhulu i-receptor yokufa (DR) i-5 mRNA okuqukethwe kumaseli, okuvumela i-Fas ligand, Fas, DR4 kanye ne-tumor necrosis factor-apoptosis-inducing ligand, (TRAIL) okuqukethwe kwe-mRNA nakho ukwanda, kepha azithintanga ama-decoy receptors (DcR) 1 kanye 2. Ukwelashwa kwe-10 μmol / L luteolin kwandise ukuvezwa kwamaprotheni e-DR5 kumaseli we-HeLa, kwaphumela ekusungulweni kweBid cleavage, ngasikhathi sinye kungasebenza i-Caspase-3, i-Caspase-8, i-Caspase-9, i-Caspase-10. Lapho amangqamuzana edluliselwa nge-DR5 ukuphazanyiswa kwe-plasmid, i-apute ye-luteolin-induction ye-HeLa cell yancishiswa kakhulu, futhi ukusebenza kwe-Caspase-9 ne-Caspase-3 kwehle. I-DR5 / Fc yomuntu ophinda ibuye ivimbele i-luteolin-indosed apoptosis. I-Luteolin induces apoptosis kumaseli e-HeLa ngomzila wokufa wokuthola, futhi i-DR5 idlala indima ebalulekile kuLuteolin indop aposis.

Le ndatshana inemininingwane yokuchazwa kukaLuteolin ngokushintshwa kwesimila, ukukhula kwesimila kanye ne-apoptosis kuyimibhalo eningiliziwe, Yini eyenziwa yi-luteolin kulesi simila, ngikholwa ukuthi wonke umuntu uyazi!