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The curve takes you to reveal the secret Lutein !


1. UV spectrum of lutein and its lutein ester

1.1 UV spectrum scan of lutein  ester extracted with acetone after reference to lutein reference  substance and methanol treated marigoldsuch as Fig.1 and Fig .2.


                                Fig.1  UV spectra of lutein  



Fig .2 UV spectra of lutein ester

From Fig.1 and Fig.2 shows, Both have the  same characteristic spectrum, the maximum absorption peak wavelength in  acetone is 446 nm.  


1.2 UV spectrum of methanol, ethanol and isopropanol extracts

UV spectra of extracting solvent with methanol ethanol and isopropanol from marigold such as Fig.3

     Fig.3  UV spectra of extracting solvent with methanol

                       ethanol and isopropanol

From Fig.3 shows the solubility of lutein  ester in methanol, ethanol, isopropanol decreases with the polarity of  the alcohol, dissolves  

Gradually increasing. It is a substance  with high alcohol solubility at 370 nm, and its main components are  syringic acid, quercetin, etc. Since the solubility of this substance in  methanol is large and the solubility of lutein ester in methanol is  small, so we chose   

1.3 Drawing of standard curve  

HPLC spectra of lutein such as : Fig.4  


                    Fig.4 HPLC spectra of lutein   


1.4 Selection of extraction conditions of Lutein ester  

Marigold flowers are pretreated with  alcohol and treated without alcohol, which has a great influence on the  extraction rate of lutein ester. The highly polar substance in the  flower hindered the dissolution of lutein ester by N-hexane. The  comparison results of the methanol-treated marigold flowers and the  alcohol-free extraction rate are shown in Fig. 5.


                      Fig.5 Effect of marigold conducted with methanol on the rate of extracting Lutein exter  


Experimental summary  

Pretreatment of marigold flowers with  methanol not only greatly accelerates the extraction rate of lutein  ester, but also obtains a higher purity of the ester, which is very  advantageous for the later saponification. This method is not only  suitable for the extraction of flowers, but also the extraction of dried  flowers.